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| High Precision Vibrating Microtome |
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| Main
Features: Integraslice is
A HIGH PRECISION VIBRATING MICROTOME designed to provide you with the
highest quality slices in the most difficult tissue slicing
applications. This is achieved with the following combination of
features:
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Precision mechanical
movement
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Electronic control system
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Precision blade
technology
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Temperature control
All models are supplied
with a standard tissue bath and holder, 50 stainless steel blades, and
two ceramic blades. |
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Fine control at your
fingertips
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10 micron per second
speed resolution for blade advance and reversing during
sectioning.
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Constant feedback
system allows cutting speed to be maintained through variations in
tissue density.
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Blade returns for next
slice at high speed to minimize total sectioning time.
The result is a minimum of induced
trauma, and maximum preservation of cell structure and biochemical
action.
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Micron control of slice
thickness
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A precision mechanical
system raises tissue against gravity between slices for maximum
accuracy.
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Increments of 1 micron
height resolution by stepper motor control.
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Viewing mirror at rear
of tissue enables you to see the point of entry.
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Coarse vertical height
adjustment of 1mm per second is also available.
Tissue is automatically withdrawn during the reverse stroke so
the blade is not drawn across the next slice.
This is illustrated below: |
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Observation during
sectioning |
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The unique ‘Clearview’ observation
window above the blade provides a ripple free surface on the
artificial cerebro-spinal fluid (a.c.s.f.). Observation can be
with the naked eye or with an integrally mounted inspection
microscope or magnifying glass. Flexible fibre optic guides of
the cold light source provide illumination where it is needed. The
Cold Light Source Model 7550LS, Magnifying Glass Model 7550MG and
Inspection Microscope Model 7550IM may be purchased as separate
options. |
‘Clearview’ observation window
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Ergonomic user interface |
- Liquid Crystal Display (LCD) with associated membrane keypad.
- Easy-to-follow menu available in multiple languages.
- Twin rotary dial knobs offer variable control of the blade
advance and return during sectioning and variable control of
brightness for the cold light source.
- Two-stage foot-switch; first starts the blade oscillating and
the second starts the advance of the blade.
- Thus, when the footswitch is released the tissue is never
pushed against a static blade.
- The enclosure gives style and cleanliness, sealing against
accidental spillage and is tolerant of salt and sugar a.c.s.f.
Easy access to change the light source bulb and the drive belts.
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Rotary dial knobs
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Model 7550PSDS
Programmable cutting
profiles of Speed, Distance and Section thickness
Features include Dynamically Adjustable
Speed and Amplitude (DASA).
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Model 7550PSDS Features:
PSDS denotes Programmable cutting profiles of Speed, Distance and
Section thickness. Using the rotary dial knob, the speed of advance
can be slowed through one area and increased through another, with a
resolution of 10 microns per second. By selecting record mode and
going manually through the first slice, the instrument effectively
learns the changes of speed and distance as the blade travels. When
the third parameter of section thickness is entered and the number
of slices required is set, the instrument will then faithfully
reproduce the parameters that it recorded. This frees the operator’s
hands enabling them to harvest the slices in the shortest possible
time.
Another feature is the DASA control. This is unique to Campden
Instruments and is available only in this model. DASA is where both
the speed of oscillation and the amplitude of the cut can be
dynamically adjusted, in steps of 0.1mm, while the blade is in
motion. The relationship between speed and amplitude is inversely
proportional, the limits of which are set by an internal control
system. |
Model
7550PSDS Shown with optional accessories:
Model
7550LS Cold Light Source
Model
7550IM Inspection Microscope
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The relationship between speed and amplitude: |
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Amplitude (mm) |
Max. Speed (Hz) |
Min. Speed (Hz) |
| 0.5 |
115 |
20 |
| 1.0 |
100 |
20 |
| 1.5
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80 |
20 |
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Microtome Accessories:
- Campden's Precision Blades in Zirconium Ceramic and Stainless
Steel
- Temperature Controlled Peltier Tissue Baths
- Cold Light Source
- Magnifying Glass or Inspection Microscope
- Autoclavable Baths
- Autoclavable Cutting Head
- Tilting Tissue Mount
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Model 7550MM
Motorized or Manual
advance of the oscillating blade
Features
include a set speed motorized advance/manual advance controlled by a
rotary dial knob.
Amplitude
of oscillation can be manually adjusted.
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Features:
MM denotes Manual or Motorized blade advance. This
model has all the fine control of the PSDS but without the
programmability or the DASA control. The amplitude is manually
adjustable between 0.5mm to 1.5mm at a maximum oscillation speed of
80Hz. The manual advance offers the fingertip sensitivity of the
rotary dial knob. The set speed advance gives repeatability and
consistency. Visually the MM and the PSDS models are similar with
the only differences appearing in the menu selections and membrane
switch user interface.
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LCD with associated membrane keypad
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Precision Microtome Blades:
Development of our blade technology has been integral to our
Microtome design. The blades are available in stainless steel and
zirconium ceramic. Due to their special shape, they fit into
Campden’s blade holder so that the tissue returns to the perfused
a.c.s.f. as quickly as possible. See pages 11 and 12 for further
details.
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Integraslice
Specifications: |
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Blade Motion: |
Semi-elliptical with minimal vertical movement |
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Oscillation Speed: |
Linear
from 20Hz to 115Hz by rotary dial knob |
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Amplitude of Cut: |
7550MM
Manually adjustable, 0.5mm to 1.5mm
7550PSDS Dynamically adjustable
with speed [DASA], 0.5mm to 1.5mm |
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Blade Advance Speed: |
From
-1.0mm/sec to + 3.0mm/sec in 10 micron steps |
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Blade Return Speed: |
4.0mm/sec (tissue is automatically lowered) |
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Section Thickness:
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1
micron resolution, retained in memory |
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Vertical Range: |
32mm
total, in 1mm steps / max speed of 1mm/sec |
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Sectioning Range: |
From
1mm to 40mm |
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Maximum Specimen
Size: |
30mm x
40mm |
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Tissue Bath: |
55mm x
82mm x 31mm /140ml, Standard, Autoclavable or Cooled |
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Cutting Head: |
Autoclavable as standard |
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Blades: |
Ceramic
7550/1/C or Stainless Steel 7550/1/SS |
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Observation: |
Inspection Microscope 10x-40x with zoom or Magnifying Glass 2x
and 'Clearview' window |
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Power Supply |
115/230VAC at 50Hz to 60Hz |
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Z-Axis
Deflection Statement...
Campden Instruments uses a method
of non-contact metrology on every Integraslice machine both
during production and in final test before dispatch. This method
measures the degree of occlusion of a photobeam by the
oscillating blade.
A statement of ‘x’ microns of
z-axis deflection by itself is not meaningful. A true
representation of performance can only be given in the context
of the speed and the amplitude of oscillation and the condition
of the surface being measured.
- Surface Measure -
The surface of Campden
Instruments Ceramic Blades, Model
7550/1/C, are first ground and then lapped flat with
micron sized diamond grit.
- Scope of Measurement -
Measurements are taken at three
different amplitudes these being 0.5mm, 1.0mm and 1.5mm and at
each amplitude from 20Hz to the maximum oscillation speed at
that amplitude.
In these conditions our
statement on z-axis deflection is as follows:
"With this evaluation Campden Instruments is able
to guarantee that at all speeds of oscillation and amplitudes
the z-axis deflection averages 4 and is not more than 8 microns"
Comparative measurements have
shown Integraslice to be an order of magnitude [i.e. 10x]
improvement on the Vibroslice. Integraslice does not require
regular servicing to maintain its z-axis accuracy due to the
mechanical design of this aspect. |
For additional information please call: 0150 9814790
View our other Slice Systems
Information:
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