Tissue Bath Coolers for Campden's Vibrating Microtomes  -  Model 7600 series

Once removed from the animal, tissue must be immediately cooled to lower the oxygen demand and thus preventing anoxia. Some workers consider that perfusing brain before removal is preferable. Cardiomyocetes are more robust to low oxygen concentrations but tissue slices better at lower temperatures

Holding the tissue close to 4 Celsius must continue throughout the slicing procedure. This can be done with passive cooling where a known amount of ice is used to maintain the cooled a.c.s.f. or with an electronically controlled Tissue Bath Cooler.

• Description

• Model range

• Application

• Technical specifications

• Order Codes

Description:

The control unit supplies power to the 'Peltier' thermoelectric elements in the base of the tissue bath. These act as energy transfer units so that heat is drawn off, through the heat exchanger and removed by tap water flow which runs to waste  The unit uses P.I.D. (proportional integral derivative) temperature control to take the bath temperature to within 0.5ºC at the point of measurement. The temperature feedback thermistor is located in the bath floor and software calculates an offset automatically.

Model  Range:

• Model 7600 standard tissue bath cooler

• Model 7660S with removable bath for sterilization by autoclaving

• Compatible with vibrating microtome models 7550PSDS, 7550MM, HA752, MA752 and ROMA752, as well as older Campden models

• Autoclavable versions have baths detachable from the thermoelectric elements

• The bath chamber has the same volume as the Standard Tissue Bath Model 752/2B and non-cooled Autoclavable Bath Model 752/2/AC

• Uses a Standard Tissue Mount Model 752/2A or Tilting Tissue Mount Model 752/2A/A

Application:

Immediate cooling of the tissue prevents anoxia by lowering the oxygen demand. Once removed from the animal tissue should be immediately place in cooled saline or sucrose buffer before mounting in the tissue bath.

Cyanoacrylate 'superglue' is readily available and is the most common method of adhering  tissue to the mount inside the bath. Cyanoacrylate polymerises on contact with water and thus a good bond is formed. Excess moisture should be removed from the tissue before placing on the blob of adhesive, then gently pour the prepared cooled buffer over the tissue.  One disadvantage of ordinary 'superglue' is that it is cytotoxic and so the lowest 2mm of tissue are lost. For this reason some workers prefer to use 'Histoacryl' brand. Sectioning at 4 Celsius improves physical preservation of tissue and viability for in-vitro recordings.

Some enzyme histochemical techniques give better staining results when sectioned at low temperatures.

Technical Specifications:

Order codes:

View our other Slice Systems Information:

• Synchroslice  - NEW!

• Integraslice

• Vibroslice ^TM

• Illumination and Observation

• Autoclavable Accessories

• Precision Microtome Blades

• Temperature Controlled Tissue Baths

• Slice and Perfusion Chambers (PID Temperature Control)

• OptoPatch Clamp Amplifier

• McIlwain Tissue Chopper

• Other Tissue Slice Models

• Decapitators

• Engineered for Quality

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